tripan blue exclusion test Search Results


tripan blue dye exclusion assay  (Millipore)
90
Millipore tripan blue dye exclusion assay
Tripan Blue Dye Exclusion Assay, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tripan blue exclusion test  (Bio-Rad)
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Bio-Rad tripan blue exclusion test
Tripan Blue Exclusion Test, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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0.25% trypsin edta  (Millipore)
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Millipore 0.25% trypsin edta
0.25% Trypsin Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tripan blue  (Nacalai)
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Nacalai tripan blue
The effects of KD-cGAS on cell proliferation was evaluated. MM231 cells <t>(DMSO,</t> <t>PTX-short,</t> ERI-short, KD-cGAS-DMSO, KD-cGAS-PTX, KD-cGAS-ERI) were used ( a ) Proliferation assay was performed. 24 h after knockdown of cGAS was defined as 0 h, and cell proliferation was evaluated every 12 h. ( b ) <t>Tripan</t> blue stain was used to evaluate the percentage of live and dead cells 24 h after treatment. ( c ) We evaluated the effects of knocking down of cGAS on RAD51 expression. Vinculin was used as loading control. ( d ) Immunofluorescence with RAD51 and γH2Ax was performed. DAPI was used for nuclear staining. The white line is 10 μm. ( e ) We counted the number of nuclear foci in each cell stained with γH2Ax and RAD51 on average. The meaning of the asterisks are as follows: *p < 0.05, **p < 0.01.
Tripan Blue, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tripan blue  (Millipore)
90
Millipore tripan blue
The effects of KD-cGAS on cell proliferation was evaluated. MM231 cells <t>(DMSO,</t> <t>PTX-short,</t> ERI-short, KD-cGAS-DMSO, KD-cGAS-PTX, KD-cGAS-ERI) were used ( a ) Proliferation assay was performed. 24 h after knockdown of cGAS was defined as 0 h, and cell proliferation was evaluated every 12 h. ( b ) <t>Tripan</t> blue stain was used to evaluate the percentage of live and dead cells 24 h after treatment. ( c ) We evaluated the effects of knocking down of cGAS on RAD51 expression. Vinculin was used as loading control. ( d ) Immunofluorescence with RAD51 and γH2Ax was performed. DAPI was used for nuclear staining. The white line is 10 μm. ( e ) We counted the number of nuclear foci in each cell stained with γH2Ax and RAD51 on average. The meaning of the asterisks are as follows: *p < 0.05, **p < 0.01.
Tripan Blue, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tripan blue staining  (Merck KGaA)
90
Merck KGaA tripan blue staining
The effects of KD-cGAS on cell proliferation was evaluated. MM231 cells <t>(DMSO,</t> <t>PTX-short,</t> ERI-short, KD-cGAS-DMSO, KD-cGAS-PTX, KD-cGAS-ERI) were used ( a ) Proliferation assay was performed. 24 h after knockdown of cGAS was defined as 0 h, and cell proliferation was evaluated every 12 h. ( b ) <t>Tripan</t> blue stain was used to evaluate the percentage of live and dead cells 24 h after treatment. ( c ) We evaluated the effects of knocking down of cGAS on RAD51 expression. Vinculin was used as loading control. ( d ) Immunofluorescence with RAD51 and γH2Ax was performed. DAPI was used for nuclear staining. The white line is 10 μm. ( e ) We counted the number of nuclear foci in each cell stained with γH2Ax and RAD51 on average. The meaning of the asterisks are as follows: *p < 0.05, **p < 0.01.
Tripan Blue Staining, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tripan blue solution  (Millipore)
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Millipore tripan blue solution
The effects of KD-cGAS on cell proliferation was evaluated. MM231 cells <t>(DMSO,</t> <t>PTX-short,</t> ERI-short, KD-cGAS-DMSO, KD-cGAS-PTX, KD-cGAS-ERI) were used ( a ) Proliferation assay was performed. 24 h after knockdown of cGAS was defined as 0 h, and cell proliferation was evaluated every 12 h. ( b ) <t>Tripan</t> blue stain was used to evaluate the percentage of live and dead cells 24 h after treatment. ( c ) We evaluated the effects of knocking down of cGAS on RAD51 expression. Vinculin was used as loading control. ( d ) Immunofluorescence with RAD51 and γH2Ax was performed. DAPI was used for nuclear staining. The white line is 10 μm. ( e ) We counted the number of nuclear foci in each cell stained with γH2Ax and RAD51 on average. The meaning of the asterisks are as follows: *p < 0.05, **p < 0.01.
Tripan Blue Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tripon  (Difco)
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Difco tripon
The effects of KD-cGAS on cell proliferation was evaluated. MM231 cells <t>(DMSO,</t> <t>PTX-short,</t> ERI-short, KD-cGAS-DMSO, KD-cGAS-PTX, KD-cGAS-ERI) were used ( a ) Proliferation assay was performed. 24 h after knockdown of cGAS was defined as 0 h, and cell proliferation was evaluated every 12 h. ( b ) <t>Tripan</t> blue stain was used to evaluate the percentage of live and dead cells 24 h after treatment. ( c ) We evaluated the effects of knocking down of cGAS on RAD51 expression. Vinculin was used as loading control. ( d ) Immunofluorescence with RAD51 and γH2Ax was performed. DAPI was used for nuclear staining. The white line is 10 μm. ( e ) We counted the number of nuclear foci in each cell stained with γH2Ax and RAD51 on average. The meaning of the asterisks are as follows: *p < 0.05, **p < 0.01.
Tripon, supplied by Difco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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urine test strips with color charts for protein, glucose, and ph triphan  (Pliva Inc)
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Pliva Inc urine test strips with color charts for protein, glucose, and ph triphan
The effects of KD-cGAS on cell proliferation was evaluated. MM231 cells <t>(DMSO,</t> <t>PTX-short,</t> ERI-short, KD-cGAS-DMSO, KD-cGAS-PTX, KD-cGAS-ERI) were used ( a ) Proliferation assay was performed. 24 h after knockdown of cGAS was defined as 0 h, and cell proliferation was evaluated every 12 h. ( b ) <t>Tripan</t> blue stain was used to evaluate the percentage of live and dead cells 24 h after treatment. ( c ) We evaluated the effects of knocking down of cGAS on RAD51 expression. Vinculin was used as loading control. ( d ) Immunofluorescence with RAD51 and γH2Ax was performed. DAPI was used for nuclear staining. The white line is 10 μm. ( e ) We counted the number of nuclear foci in each cell stained with γH2Ax and RAD51 on average. The meaning of the asterisks are as follows: *p < 0.05, **p < 0.01.
Urine Test Strips With Color Charts For Protein, Glucose, And Ph Triphan, supplied by Pliva Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tac film triphan  (Lonza)
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Lonza tac film triphan
The effects of KD-cGAS on cell proliferation was evaluated. MM231 cells <t>(DMSO,</t> <t>PTX-short,</t> ERI-short, KD-cGAS-DMSO, KD-cGAS-PTX, KD-cGAS-ERI) were used ( a ) Proliferation assay was performed. 24 h after knockdown of cGAS was defined as 0 h, and cell proliferation was evaluated every 12 h. ( b ) <t>Tripan</t> blue stain was used to evaluate the percentage of live and dead cells 24 h after treatment. ( c ) We evaluated the effects of knocking down of cGAS on RAD51 expression. Vinculin was used as loading control. ( d ) Immunofluorescence with RAD51 and γH2Ax was performed. DAPI was used for nuclear staining. The white line is 10 μm. ( e ) We counted the number of nuclear foci in each cell stained with γH2Ax and RAD51 on average. The meaning of the asterisks are as follows: *p < 0.05, **p < 0.01.
Tac Film Triphan, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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triptan medications  (IQVIA Inc)
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IQVIA Inc triptan medications
The effects of KD-cGAS on cell proliferation was evaluated. MM231 cells <t>(DMSO,</t> <t>PTX-short,</t> ERI-short, KD-cGAS-DMSO, KD-cGAS-PTX, KD-cGAS-ERI) were used ( a ) Proliferation assay was performed. 24 h after knockdown of cGAS was defined as 0 h, and cell proliferation was evaluated every 12 h. ( b ) <t>Tripan</t> blue stain was used to evaluate the percentage of live and dead cells 24 h after treatment. ( c ) We evaluated the effects of knocking down of cGAS on RAD51 expression. Vinculin was used as loading control. ( d ) Immunofluorescence with RAD51 and γH2Ax was performed. DAPI was used for nuclear staining. The white line is 10 μm. ( e ) We counted the number of nuclear foci in each cell stained with γH2Ax and RAD51 on average. The meaning of the asterisks are as follows: *p < 0.05, **p < 0.01.
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imitrex® nasal spray  (Glaxo Smith)
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Glaxo Smith imitrex® nasal spray
The effects of KD-cGAS on cell proliferation was evaluated. MM231 cells <t>(DMSO,</t> <t>PTX-short,</t> ERI-short, KD-cGAS-DMSO, KD-cGAS-PTX, KD-cGAS-ERI) were used ( a ) Proliferation assay was performed. 24 h after knockdown of cGAS was defined as 0 h, and cell proliferation was evaluated every 12 h. ( b ) <t>Tripan</t> blue stain was used to evaluate the percentage of live and dead cells 24 h after treatment. ( c ) We evaluated the effects of knocking down of cGAS on RAD51 expression. Vinculin was used as loading control. ( d ) Immunofluorescence with RAD51 and γH2Ax was performed. DAPI was used for nuclear staining. The white line is 10 μm. ( e ) We counted the number of nuclear foci in each cell stained with γH2Ax and RAD51 on average. The meaning of the asterisks are as follows: *p < 0.05, **p < 0.01.
Imitrex® Nasal Spray, supplied by Glaxo Smith, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The effects of KD-cGAS on cell proliferation was evaluated. MM231 cells (DMSO, PTX-short, ERI-short, KD-cGAS-DMSO, KD-cGAS-PTX, KD-cGAS-ERI) were used ( a ) Proliferation assay was performed. 24 h after knockdown of cGAS was defined as 0 h, and cell proliferation was evaluated every 12 h. ( b ) Tripan blue stain was used to evaluate the percentage of live and dead cells 24 h after treatment. ( c ) We evaluated the effects of knocking down of cGAS on RAD51 expression. Vinculin was used as loading control. ( d ) Immunofluorescence with RAD51 and γH2Ax was performed. DAPI was used for nuclear staining. The white line is 10 μm. ( e ) We counted the number of nuclear foci in each cell stained with γH2Ax and RAD51 on average. The meaning of the asterisks are as follows: *p < 0.05, **p < 0.01.

Journal: Scientific Reports

Article Title: Eribulin induces micronuclei and enhances the nuclear localization of cGAS in triple-negative breast cancer cells

doi: 10.1038/s41598-024-64651-y

Figure Lengend Snippet: The effects of KD-cGAS on cell proliferation was evaluated. MM231 cells (DMSO, PTX-short, ERI-short, KD-cGAS-DMSO, KD-cGAS-PTX, KD-cGAS-ERI) were used ( a ) Proliferation assay was performed. 24 h after knockdown of cGAS was defined as 0 h, and cell proliferation was evaluated every 12 h. ( b ) Tripan blue stain was used to evaluate the percentage of live and dead cells 24 h after treatment. ( c ) We evaluated the effects of knocking down of cGAS on RAD51 expression. Vinculin was used as loading control. ( d ) Immunofluorescence with RAD51 and γH2Ax was performed. DAPI was used for nuclear staining. The white line is 10 μm. ( e ) We counted the number of nuclear foci in each cell stained with γH2Ax and RAD51 on average. The meaning of the asterisks are as follows: *p < 0.05, **p < 0.01.

Article Snippet: The cell lysates from MM231 cell lines (DMSO, PTX-short, ERI-short, KD-cGAS-DMSO, KD-cGAS-PTX, KD-cGAS-ERI), which were 24 h after PTX or ERI treatment, were extracted and diluted equally by tripan blue (Nacalai, #20577-34).

Techniques: Proliferation Assay, Knockdown, Staining, Expressing, Control, Immunofluorescence